5'-UTR:
5' -untranslated region derived from human alpha-globin RNA with an optimized Kozak sequence
ORF:
Glutathione-S transferase (GST)
3'-UTR:
3' UTR comprising two sequence elements derived from the aminoterminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA
Poly(A) Tail:
A 110-nucleotide poly(A)-tail consisting
of a stretch of 30 adenosine residues,
followed by a 10-nucleotide linker
sequence and another 70 adenosine
residues.
Modifications:
N1-methyl-pseudouridine
Neutral Lipid:
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)
Lonizable Lipid:
1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2000-DMG)
PEG-lipid:
Heptadecan-9-yl 8-((2-hydroxyethyl)(8-(nonyloxy)− 8-oxooctyl)amino)octanoate)(SM-102)
Cryoprotectant:
Trehalose
