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Renilla luciferase(Rluc) In Vitro Transcribed mRNA-LNP
MRNA-TG-009
Renilla luciferase(Rluc) In Vitro Transcribed mRNA-LNP
Source:In vitro transcribed mRNA encapsulated with LNP
SKU:MRNA-TG-009-DCPS
Product Name:Cap1-Renilla luciferase(Rluc)-120nt PolyA Tail In Vitro Transcribed mRNA
Product Description:In Vitro Transcribed mRNA encoding Renilla luciferase(Rluc)ORF with Cap1 and 120nt PolyA tail.
SKU:MRNA-TG-009-DCPS
Product Name:Cap1-Renilla luciferase(Rluc)-120nt PolyA Tail In Vitro Transcribed mRNA
Product Description:In Vitro Transcribed mRNA encoding Renilla luciferase(Rluc)ORF with Cap1 and 120nt PolyA tail.
PROPERTIES
Cap:
Cap 1
5'-UTR:
5' -untranslated region derived from human alpha-globin RNA with an optimized Kozak sequence
ORF:
Renilla luciferase(Rluc)
3'-UTR:
3' UTR comprising two sequence elements derived from the aminoterminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA
Poly(A) Tail:
A 110-nucleotide poly(A)-tail consisting of a stretch of 30 adenosine residues, followed by a 10-nucleotide linker sequence and another 70 adenosine residues.
Modifications:
N1-methyl-pseudouridine
Neutral Lipid:
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)
Cholesterol:
Cholesterol
Lonizable Lipid:
1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2000-DMG)
PEG-lipid:
Heptadecan-9-yl 8-((2-hydroxyethyl)(8-(nonyloxy)− 8-oxooctyl)amino)octanoate)(SM-102)
Storage:
-20 °C
Buffer:
PBS, pH7.4
Cryoprotectant:
Trehalose
Related Categories
Reporter Gene mRNA
Background

Background

Renilla luciferase (RLuc) is commonly used as a reporter gene either on its own, or in conjunction with firefly and other beetle luciferases. It can be used in combination with the firefly luciferase as a dual luceriferase reporter gene system. The pre-packaged lentiviral particles can be transduced into any mammalian cell type for constitutively and stably expressing the Renilla luciferase reporter gene. Multiple choices of antibiotic stable-selection markers are available.

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