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SUMO In Vitro Transcribed mRNA-LNP
MRNA-TG-006
SUMO In Vitro Transcribed mRNA-LNP
Source:In vitro transcribed mRNA encapsulated with LNP
SKU:MRNA-TG-006-DCPS
Product Name:Cap1-SUMO-120nt PolyA Tail In Vitro Transcribed mRNA
Product Description:In Vitro Transcribed mRNA encoding SUMOORF with Cap1 and 120nt PolyA tail.
SKU:MRNA-TG-006-DCPS
Product Name:Cap1-SUMO-120nt PolyA Tail In Vitro Transcribed mRNA
Product Description:In Vitro Transcribed mRNA encoding SUMOORF with Cap1 and 120nt PolyA tail.
PROPERTIES
Cap:
Cap 1
5'-UTR:
5' -untranslated region derived from human alpha-globin RNA with an optimized Kozak sequence
ORF:
SUMO
3'-UTR:
3' UTR comprising two sequence elements derived from the aminoterminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA
Poly(A) Tail:
A 110-nucleotide poly(A)-tail consisting of a stretch of 30 adenosine residues, followed by a 10-nucleotide linker sequence and another 70 adenosine residues.
Modifications:
N1-methyl-pseudouridine
Neutral Lipid:
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)
Cholesterol:
Cholesterol
Lonizable Lipid:
1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (PEG2000-DMG)
PEG-lipid:
Heptadecan-9-yl 8-((2-hydroxyethyl)(8-(nonyloxy)− 8-oxooctyl)amino)octanoate)(SM-102)
Storage:
-20 °C
Buffer:
PBS, pH7.4
Cryoprotectant:
Trehalose
Related Categories
Reporter Gene mRNA
Background

Background

SUMO is short for Small Ubiquitin-like Modifier, the 100 amino acid sequence, which is necessary for regulation of protein transport and is important for controlling transcription for eukaryotic cells. Recombinant proteins expressed in E. coli may fail to fold properly, instead forming aggregates and precipitating as inclusion bodies. This insolubility may be due to the presence of codons read inefficiently by E. coli, differences in eukaryotic and prokaryotic ribosomes, or lack of appropriate molecular chaperones for proper protein folding. In order to purify such proteins it may be necessary to fuse the protein of interest with a solubility tag such as SUMO or MBP (maltose-binding protein) to increase the protein's solubility. SUMO can later be cleaved from the protein of interest using a SUMO-specific protease such as Ulp1 peptidase.

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