Characterization of Liposomes by Capillary Electrophoresis (CE)

CE, short for capillary electrophoresis, is a purity measurement utilizing a gel filled capillary to assess the proportion of full-length products to truncated products present in the final oligo sample. Capillary electrophoresis (CE) is a flexible method that gives speedy separations with top-notch performance and resolution. Seattle Genova offers variety of modern CE and capillary electrophoresis mass spectrometry (CE/MS) instrumentation gives a flexible and efficient alternative to LC for difficult analytical challenges. We are providing our customers, all physical parameters for characterization of liposomes

CE is an analytical technique based on the separation principles; the analytes are separated under the influence of the applied voltage, according to their charge, mass, and structure. Separation of tested compounds in the capillary is an effect of two phenomena: electrophoretic migration (migration of charged particles in the electric field toward the oppositely charged electrode) and electroosmotic flow (EOF). Capillary electromigration methods are suitable for the study of liposomes, because they exhibit several advantageous features, such as low sample consumption, fast and efficient separations, and a high degree of automation. This is the reason, it is highly suitable for characterizing the drug–liposome interactions, for determination of encapsulation efficiency, for studying the drug leakage from liposomes, as well as for liposomes characterization (e.g., size, surface charge, and permeability of the phospholipid membrane). As an electrophoretic tool it is characterized also by simplicity, high separation efficiency, economical use of solvents and environment-friendly nature – goals hard to achieve in case of many LC-based methods. Moreover, unlike HPLC and IAM-HPLC chromatographic stationary phases, the CEC liposomal layer mimicking BBB preserves the bilayer structure, characteristic for natural biological membranes.

Deliverables

• A complete high quality CE service for the characterization of liposomes (your sample).

• A complete work report.