Characterization of Liposomes by Size Exclusion Chromatography (SEC)

For polymer, liposomes and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Seattle Genova gives a variety of SEC systems or individual SEC detectors to fit your utility needs. With the help of our experts’ knowledge and experiences, Seattle Genova provides complete hassle free services on the characterization of liposomes through size exclusion Chromatography (SEC). 

Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel. Conventional size exclusion chromatography (SEC), and high performance size exclusion chromatography (HPSEC) are techniques mainly thrilling as they allow to physically separate liposomes from small solutes, on the one hand, and to subdivide liposomes into a series of subpopulations, on the other hand. This lets in specific determination of size and size distribution. In addition, as this approach is not destructive for the samples, fractions after collection may be essayed for various complementary analyses. Size exclusion chromatography is a simple and powerful technique for investigation of encapsulation, insertion/interaction of substances from small solutes (ions, surfactants, drugs, etc.) up to large molecules (proteins, peptides and nucleic acids) in liposomes. When SEC is coupled with light scattering, viscometer and concentration detectors (referred to as triple detection), it is able to measure absolute molecular weight, molecular size, and intrinsic viscosity and generate records on macromolecular structure, conformation, aggregation and branching. By using SEC to measure molecular weight and these other properties, scientists can characterize molecules which includes proteins, synthetic polymers, and natural polymers including polysaccharides. For all of those molecules, those parameters relate very carefully to their overall performance in endless applications.

Deliverables

• Complete SEC service for the characterization of your sample.

• A complete work report.