Characterization of Liposomes with Zeta Potential (ζ-Potential)

Seattle Genova has the best approach for analysis and characterization of liposomes. Seattle Genova have comprehensive equipment in our analysis center. In order to precisely test the stability of liposomes, we are equipped with the high quality, good precision analyzer for zeta potential measurement. We offers comprehensive high quality, hassle free, cost friendly services on the liposomes formulation and characterization to fulfil research needs for our customers.

In Seattle Genova, we use zeta analyzer to determine charge distribution on liposomes. Zeta potential is the potential difference among the dispersion medium and the stationary layer of fluid attached to the dispersed particle. It regulates the following liposomes’ factors:

• Colloidal stability

• Their biodistribution

• Pharmacokinetics

• Cellular affinity

• Drug-internalization processes

Mechanism

A liposome nanocarrier scattered in an aqueous solution gains a surface charge as a result of either the ionization of surface end-groups or the adsorption of charged species on its surface. The dispersed counter-ions will encircling the surface of the particle, at the same time as the zeta potential determines the electrical potential on the nanoparticle interface. The zeta potential is a primary detector of the colloidal stability of a liposome’s dispersions, because it expresses the degree of electrostatic repulsion among nanoparticles in a dispersion.

Determining factors

• If zeta potential of nanoparticles is high, then it shows they are electrically stabilized.

• But if zeta potential is low, then they are either aggregated or flocculated.

Furthermore, phase analysis light scattering (PALS) techniques is used to measure electrophoretic mobility µE of colloidal nanoparticles. Zeta potential and PALS techniques used as a combination to analyze electrostatic interactions in charged liposomes. This could further help in investigating the interaction present between complex charges liposomes and target cells or tissues.

Deliverables

• A complete zeta potential (ζ-Potential) analysis on your sample.

• A complete work report.