Drug Entrapment Efficiency

Seattle Genova, with help of PHD experts, has developed wide range of services for the characterization of liposomes. We offer not only one but all different physical parameters to analyze your sample. We have excellent technique for liposome design and manufacture, but we have also possessed a comprehensive set of equipment for liposome analysis. We can offer accurate analysis service for drug entrapment efficiency for our customer’s sample to fulfil their research needs. Seattle Genova offers reliable service for liposome characterization. We are specialized to design and develop custom analytical method suitable for the characterization (e.g., encapsulation efficiency) of specific samples.

The encapsulation efficiency is the percentage of drug that is successfully entrapped into liposomes. It is defined by the concentration of the incorporated material detected in the formulation over the initial concentration used to make the formulation. Encapsulation efficiency is one of the most important parameters for liposome formulation as it directly reflects the concentration of active ingredients (drugs, proteins, antimicrobial agents, etc.). Factors affecting the encapsulation efficiency of the drug depend on properties of both the liposome and encapsulated drugs. Concerning the encapsulated drugs, the encapsulation efficiency is affected by their hydrophilicity or lipophilicity. A number of liposome properties, such as aqueous volume, membrane rigidity, and surface area and preparation methods are reported to have influence on the encapsulation efficiency.

Steps for DEE

In order to calculate encapsulation efficacy.

1.      Separate non-entrapped drug from drug-loaded liposomes. Centrifuge and exclusion column have been used widely for separation.

2.      The concentration of drugs can be detected using selected method according to their properties by high-performance liquid chromatography (HPLC), circular dichroism (CD) spectroscopy.