Characterization of Liposomes by High Performance Liquid Chromatography (HPLC)

With all of the series of offerings on the characterization of lipids, Seattle Genova is here to offer services of HPLC analysis of lipids to cover all aspects of characterization. Seattle Genova ensures its customers get all the solutions for their problems, here at the same place to overcome the burden of their research needs. With our expert help, our customers will be provided with high-quality, cost-friendly, and precise analysis of lipids for their research.

Methods

The HPLC technique has been broadly implemented for the determination of drugs in liposome formulation. Some of those strategies contain the evaluation of encapsulated drugs in liposomes or drugs encapsulated in liposomes in biological samples including plasma and liver. The HPLC techniques for the control of liposomal formulations are based on adjusting parameters along with mobile phase and flow speed suspension can be ranged from translucent to milky, depending on the composition and particle size. In contrast, a non-liposomal drug is retained on the stationary phase that is subsequently determined through high-pressure liquid chromatography (HPLC) or electrophoresis.

Solid-phase extraction (SPE): It is significantly used to separate liposomal and non-liposomal drug forms. Separation is based on the property of liposomes to cross reversed-phase C18 silica gel cartridges without being retained.

UV, fluorimetric, and mass spectrometry (MS): These detection modes, significantly utilized in HPLC of drug-delivery liposomes.

Dynamic light scattering: It is used to analyze size distribution of liposomes.

Electron microscopy or by spectroscopic method: It measures the lamellarity of liposomes.

Furthermore, stable liposomes formation preserves its size distribution that results in physical stability. While, if drug leaks from vesicle or aggregates with vesicles to form large particles, then it leads to physical instability.