Liposome Production by Sonication Method

Seattle Genova has spent years developing liposome technologies, and our extensive experience qualifies us as experts in liposome preparation and manufacturing. Our liposome platform's goal is to provide you with high-quality liposome services ranging from custom liposome production, analysis, and characterization to application.

Service Highlights

• Varied types of liposome-based drug delivery systems

• Controlled/sustained release drug delivery system

• Pre-formulation, formulation feasibility and prototype development

• A combination of mature preparation technologies are available

• Different identification and standardization methods

• Procedure optimization (experimental design) and aseptic filtration

Sonication is a an easy and efficient technique to reduce particle sizes of liposomes. The cavitation events caused by sonication can break up larger, multilamellar lipid vesicles and turn them into smaller vesicles.

Sonication can be utilized for the production of nanoparticles, such as nanoemulsions, nanocrystals, liposomes and wax emulsions, as well as for wastewater purification, degassing, extraction of seaweed polysaccharides and plant oil, extraction of anthocyanins and antioxidants,  generation of biofuels, crude oil desulphurization, cell disruption, polymer and epoxy processing, adhesive thinning, and numerous other processes. It is used in pharmaceutical, cosmetic, water, food, ink, paint, coating, wood treatment, metalworking, nanocomposite, pesticide, fuel, wood product and numerous other industries.

The sonication technique is established on size transformation and involves the subsequent sonication of MLVs formulated by thin-film hydration method, utilizing sonic energy usually under an inert atmosphere including nitrogen or argon. The sonication method facilitates homogenous dispersion of small vesicles utilizing bath type or probe type sonicator with a potential for greater tissue penetration. The probe tip sonicator provides high energy to the lipid suspension. The possibility of overheating of the lipid suspension induces degradation. Sonication tips manage to release titanium particles into the lipid suspension which must be removed by centrifugation prior to utilize. The bath sonicators are the greatly widely utilized instrumentation for preparation of SUV. They are seed for big volume of dilute lipids.

The oxidation of unsaturated bonds in the fatty acid chains of phospholipids and hydrolysis to lysophospholipids and free fatty acids, as well as denaturation of thermolabile substances and very low encapsulation efficiency of internal volume are the main disadvantages of the technique.

Featured Services

Non-toxicity, biocompatible, and completely biodegradable

Boosting drug efficacy

Increasing stability

Decreasing the toxicity of drugs

Flexibility for active targeting