Lipid Nanoparticles For mRNA Delivery
Liposome Production Services
Liposomes Production by Freeze-Thaw Method

Liposomes Production by Freeze-Thaw Method

Seattle Genova has spent years developing liposome technologies, and our extensive experience qualifies us as experts in liposome preparation and manufacturing. Our liposome platform's goal is to provide you with high-quality liposome services ranging from custom liposome production, analysis, and characterization to application.

Multilamellar vesicle (MLV) has a low encapsulation ability since it is relatively heterogeneous both in size and lamellarity. Freeze-thawing technique is usually used to improve the encapsulation capacity of MLV by reducing its lamellarity. Freeze-thawing method is conducted by submerging liposome sample under water and changing temperatures with large gradients.

Elimination of the solutes from the ice stage during freezing leads to the concentrated entrapment in the residual fluid. Electrostatic interaction between the lipids with negative charge and the solution with positive charge consequences in vesicle formation. Liposome can be subjected to numerous freeze-thawing cycles at a temperature above the phase transition temperature (Tm) to further enhance the encapsulation efficiency.

The ionic strength of the medium and the concentration of phospholipids both affect the freeze-thaw procedure. It alters the lamellar structure physically, which results in the production of unilamellar vesicles. The unilamellar vesicles undergo  rapid freezing, sluggish thawing, and multiple cycles of freezing and thawing. Using phospholipids dimyristoylphosphatidylcholine and distearoylphosphatidylcholine in phosphate buffered saline buffer, six freeze-thaw cycles are used to create the liposomal propranolol formulation.


Advantages and Applications of Freeze Thawing Method

  • To increase the confined volume of liposomal preparations, the freezing and thawing technique is introduced.

  • Freeze-thaw cycling is an a significant processing step in the preparation of liposomes that directs to the encapsulation of drug molecules. This technique is improve the encapsulation capacity of MLV by reducing its lamellarity.

  • The freeze-thawing process is typically used to reduce the lamellarity of MLV.

  • A maximum size was reached after five freeze-thaw cycles when liposomes were repeatedly frozen and thawed.


Features of our Services

Seattle Genova offers a number of technologies, including thin-film, sonication, extrusion, and reverse phase evaporation and freeze-thawing techniques for creating customised liposomes. Seattle Genova is a trustworthy and qualified business partner for customers in the academic or industrial fields. Contact us at any time for a thorough quote.


Service Highlights

  • Varied types of liposome-based drug delivery systems

  • Controlled/sustained release drug delivery system

  • Pre-formulation, formulation feasibility and prototype development

  • A combination of mature preparation technologies are available

  • Different identification and standardization methods

  • Procedure optimization (experimental design) and aseptic filtration



1.       C. V. Kulkarni, et al. (2016). Effect of fullerene on the dispersibility of nanostructured lipid particles and encapsulation in sterically stabilized emulsions. Journal of Colloid and Interface Science480(15): 69-75.

2.       N. J. Alves, et al. (2016). Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles. Scientific Reports6: 24866.

3.       D. Cipolla, et al. (2016). Formation of drug nanocrystals under nanoconfinement afforded by liposomes. RSC Advances6: 6223-6233.


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