Lipid Nanoparticles For mRNA Delivery
Liposome Characterization Services
Physical Characterization of Liposomes

Physical Characterization of Liposomes

Seattle Genova is the worldwide provider of life sciences’ products and offers services on wide range of productions. With the years of experience, in Seattle Genova, our scientists have not only developed mRNA preparation services but also providing LNP formulation and characterization. Seattle Genova also offers services on physical analysis for the characterization of liposomes.

The control of physical parameters is based on measuring vesicle shape, surface morphology, mean vesicle size and size distribution, surface charge, electrical surface potential and surface pH, lamellarity, phase behavior, percent of free drug/percent capture and drug release. The visible appearance of the liposome suspension may be ranged from translucent to milky, relying on the composition and particle size. The determination of liposomal size distribution is commonly measured through dynamic light scattering while the lamellarity of liposomes is measured through electron microscopy or by spectroscopic method. Physical stable liposomal formulations maintain liposome size distribution. Physical instability can be due to drug leakage from the vesicles and/or aggregation or fusion of vesicles to form large particles.


Lists of physical parameters

  • Drug Entrapment Efficiency: To determine the percentage of drug that is successfully entrapped into liposomes.

  • High Performance Liquid Chromatography (HPLC): The determination of medications in liposome formulation.

  • Size Exclusion Chromatography (SEC): To determine fractionation of liposomes.

  • High-Performance Thin-Layer Chromatography (HPTLC): To determine quantitative analysis of liposomes.

  • Capillary Electrophoresis (CE): To determine membrane fluidity and stiffness, phospholipid distribution in the membrane, membrane disruption, size distribution, and surface charge density.

  • In Vitro Drug Release Study: Dialysis technique is used for In Vitro Drug Release Studies.

  • Zeta Potential (ζ-Potential): To characterize the surface charges of microspheres.

  • Differential Scanning Calorimetry (DSC) Study: To determine phase transition temperature of phospholipids sample.

Drug Entrapment Efficiency
The encapsulation efficiency is the percentage of drug that is successfully entrapped into liposomes. It is defined by the concentration of the incorporated material detected in the formulation over the initial concentration used to make the formulation.
Characterization of Liposomes by Size Exclusion Chromatography (SEC)
With the help of our experts’ knowledge and experiences, Seattle Genova provides complete hassle free services on the characterization of liposomes through size exclusion Chromatography (SEC).
Characterization of Liposomes by Capillary Electrophoresis (CE)
Seattle Genova offers variety of modern CE and capillary electrophoresis mass spectrometry (CE/MS) instrumentation gives a flexible and efficient alternative to LC for difficult analytical challenges. We are providing our customers, all physical parameter
Characterization of Liposomes with Zeta Potential (ζ-Potential)
In order to precisely test the stability of liposomes, we are equipped with the first-in-class analyzer for zeta potential measurement.
Characterization of Liposomes by Differential Scanning Calorimetry (DSC)
DSC is a powerful analytical tool for characterizing the stability of proteins and other biomolecules directly in its native form with the aid of suitable thermodynamic models.
Characterization of Liposomes by High Performance Liquid Chromatography (HPLC)
With all of the series of offerings on the characterization of lipids, Seattle Genova is here to offer services of HPLC analysis of lipids to cover all aspects of characterization.
Characterization of Liposomes by High-Performance Thin-Layer Chromatography (HPTLC)
Our scientists has developed a precise, high quality, and cost effective HPTLC to fulfil our customer’s research needs.
Characterization of Liposomes by In Vitro Drug Release Study
Seattle Genova offers in vitro drug release study services in the category of physical characterization of liposomes. We are here to serve our customers precise records with high quality services to share the burden of their research needs.

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