Physical Characterization of Liposomes

Seattle Genova is the worldwide provider of life sciences’ products and offers services on wide range of productions. With the years of experience, in Seattle Genova, our scientists have not only developed mRNA preparation services but also providing LNP formulation and characterization. Seattle Genova also offers services on physical analysis for the characterization of liposomes.

The control of physical parameters is based on measuring vesicle shape, surface morphology, mean vesicle size and size distribution, surface charge, electrical surface potential and surface pH, lamellarity, phase behavior, percent of free drug/percent capture and drug release. The visible appearance of the liposome suspension may be ranged from translucent to milky, relying on the composition and particle size. The determination of liposomal size distribution is commonly measured through dynamic light scattering while the lamellarity of liposomes is measured through electron microscopy or by spectroscopic method. Physical stable liposomal formulations maintain liposome size distribution. Physical instability can be due to drug leakage from the vesicles and/or aggregation or fusion of vesicles to form large particles.

Lists of physical parameters

• Drug Entrapment Efficiency: To determine the percentage of drug that is successfully entrapped into liposomes.

• High Performance Liquid Chromatography (HPLC): The determination of medications in liposome formulation.

• Size Exclusion Chromatography (SEC): To determine fractionation of liposomes.

• High-Performance Thin-Layer Chromatography (HPTLC): To determine quantitative analysis of liposomes.

• Capillary Electrophoresis (CE): To determine membrane fluidity and stiffness, phospholipid distribution in the membrane, membrane disruption, size distribution, and surface charge density.

• In Vitro Drug Release Study: Dialysis technique is used for In Vitro Drug Release Studies.

• Zeta Potential (ζ-Potential): To characterize the surface charges of microspheres.

• Differential Scanning Calorimetry (DSC) Study: To determine phase transition temperature of phospholipids sample.