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The tTA dependent and rtTA dependent expression systems are two complimentary control circuits that make up the Tet Technology. They are currently referred to as the Tet-On System and the Tet-Off System, respectively (i. e. rtTA dependent). To stimulate the expression of the gene, a recombinant tetracycline-controlled transcription factor (tTA or rtTA) interacts with the tTA/rtTA responsive promoter Pet.
Tetracycline or one of its derivatives, an effector agent, controls expression. Tetracyclines work by inhibiting the transcription factors tTA and rtTA from binding to DNA. Tetracycline ligand is required by rtTA for DNA binding and subsequently transcription. Tetracycline, on the other hand, effectively prevents the interaction between tTA and DNA. As a result, the two Tet systems react to tetracyclines extremely differently, which makes them complementary because each system has distinct qualities and advantages.
◇Both expression systems are characterized by their excellent dynamic range, which enables the regulation of gene expression over several orders of magnitude without interfering with the physiology of the host cell. High induction levels in combination with low basal background expression produce an excellent dynamic range. The rtTA transcription factor is bound by tetracycline, enabling it to bind DNA at the promoter. Tetracycline stimulates the expression of genes.
◇Tet-On 3G systems exhibit better sensitivity to doxycycline (Dox) induction and reduced basal expression (by 5- to 20-fold), which is beneficial for in vivo research in tissues where high Dox concentrations are challenging to achieve (e.g., brain). For the Tet-On 3G system, we provide several plasmids and viral delivery options, as well as promoters that enable reliable long-term expression of the Tet transactivator.
◇The Tet-On mechanism enables dox to activate gene expression. These bacterial-derived systems have seen major advancements since the original Tet-conception system and development to function in eukaryotic cells.
Design of Tet-On systems
Both of these elements are offered by Tet-One systems on a single vector. The cloned gene of interest is expressed from the TRE3GS promoter (PTRE3GS) in the reverse orientation, whereas the Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 promoter (PhPGK) in the forward direction.
Features of Our Services
◆Flexible modification approaches available
◆Professional scientists and technicians with extensive experience
◆Comprehensive understanding of engineering of Tet-On system.
◆Quick completion of any project.
◆Good quality, yields, concentrations, and purity.
◆Confirmation of the accuracy
◆A more reasonable cost
※The modified lentivirus is also shipped to our customers for them to test internally.
※The customer is sent the entire samples in some phases.
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