Antibody Affinity And Kinetics Characrization

Determination of mAb binding kinetics (ka & kd) and affinity (KD) are essential to optimum drug candidate selection. The Octet system incorporates biosensors, reagents, and assay kits to enable us to perform high-throughput, real-time, label-free affinity and kinetic characterizations on a range of sample types including antibodies, proteins, and small molecules. Seattle Genova’s highly experienced team and proven protocols deliver analyses based on solid scientific data, enabling our clients developing assays, diagnostic test kits and therapeutic products to make rapid decisions confidently.

Seattle Genova provides the following antibody-related services using the OctetRed96e system:

• Isotyping determination of antibody (IgG1, IgG2a etc.)

• Quantification of IgG concentration in samples

• Monoclonal antibody screening against label-free antigen in solution

• Antibody pairing by “epitope binning” to identify and group different antibodies (to enable antibody pairing according epitope binding on the same antigen)

• Full kinetic analysis/ ranking by on rates (ka), off rates (kd), and affinity constants (KD)

Seattle Genova’s services are tailored for each project to ensure that the objectives are met or exceeded. Experienced project teams are assigned to each study focusing on progressing projects through to results in the minimum amount of time. Our clients widely regard us as professional and attentive partners who deliver quality results.