Antibiotic Switching Service

Seattle Genova offers Antibiotic Gene Switching Services to our customers. Starting from any vector, we can either add an antibiotic gene to the vector or we can switch the existing antibiotic gene to another one. 

Many plasmids are designed to include an antibiotic resistance gene, which when expressed, allows only plasmid-containing bacteria to grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple generations. 

Below you will find a few antibiotics commonly used in the lab and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic(s) to add to your LB media or LB agar plates.

Delivables

• 10 μg of engineered plasmid

• Project report including primer design and reporter sequences and cloning methods

• Sequencing data