BioActive™ Cancer Protein Production Services

Seattle Genova offers our customers excellent protein production services that keep the bioactivities of your proteins just as they were in nature. To achieve this, we can offer both nature protein purification services and recombinant protein production services. We have developed very specialized protocols to make sure the produced proteins will maintain their biological activities. And our well-developed bioassays can ensure comprehensive activity validations before we release the proteins.

Cancer is a category of diseases in which abnormal cells divide without control and can invade other tissues. Cancer cells can spread to other pieces of the body through the blood and lymph systems. Generally, cell proliferation is under tight control through many mechanisms. For instance, DNA damage and repair mechanisms occur to decrease the likelihood of genetic mutation and cell transformation. Apoptosis is required to destroy cells that characterize a threat to the integrity of the organism. In expansion, the immune system is willing to recognise and destroy cancerous cells. Nonetheless, these error-correction techniques often fail in small ways, particularly in environments that make errors more likely to occur and propagate. Accumulating disruptions in these control mechanisms direct to progressive error accumulation until unregulated proliferation and cancer forming.

Services Workflow

Our service workflow begins with you. We can give you an overview of the improvement workflow to better understand the service procedure. If you have any queries about the project, please contact us at any time.

❖Gene synthesis and Cloning 

We do sequence analysis and codon optimization for the expression system that will be used. The designed sequences are then synthesized to generate a DNA template The target gene is cloned into expression vectors for the mammalian, insect or E.coli cells.

❖Bioactivity Testing

We can develop bioassays to validate the activity of the proteins we prepared. Only those proved to be active are qualified for release. Otherwise, we will start over to try different protocols until the bioactive protein is prepared.

❖Purification 

The expression vectors are prepared with eendotoxin-freeplasmid extract kits and are transfected into 2 liters of E. coli or 500 ml of mammalian or insect suspension cells. After the cell culture period, the target protein is purified via affinity chromatography. The concentration and purity of the purified protein are determined using a bicinchoninic acid (BCA) assay and SDS-PAGE with Coomassie blue staining, respectively. The protein is desalted into 0.2 µm filtered 1x phosphate-buffered saline (PBS), pH 7.4. The protein can then be aliquoted, lyophilized, and labeled upon request.

Why you choose us?

Deliverables

√SDS-PAGE Data

√WB data

√The bioactivity Long-term data 

√The purified proteins are also shipped to our customers for them to test internally

References

1.Targeted Cancer Therapies". cancer.gov. National Cancer Institute. 26 February 2018. Retrieved 28 March 2018.

2.Defining Cancer". National Cancer Institute. 17 September 2007. Retrieved 28 March 2018.