BioActive™ Epigenetic Protein Production Services

Seattle Genova’s BioActiveTM platform is aiming to offer our customers with excellent protein production services that keep the bioactivities of your proteins just as what they were in nature. To achieve this, we can offer both nature protein purification services and recombinant protein production services. We have developed very specialized protocols to make sure the produced proteins will maintain their biological activities. And our well-developed bioassays can ensure comprehensive activity validations before we release the proteins. Working with a variation of protein expression systems, we are able to quickly produce bespoke protein reagents to fulfill customer needs. Backed by years of expertise, we have successfully produced of a variety of proteins for many biological studies.

Epigenetics relates to heritable cellular and physiological traits which are not related to changes in DNA sequences. Although each cell includes the same DNA sequences, epigenetic regulation controls the correct transcriptional pattern in a given cell group. Thus, epigenetic regulation plays a vital role in cell proliferation, differentiation and development.

Epigenetic modifications comprise histone modification, DNA methylation and RNAi. Post-translational modifications of histone proteins include histone acetylation, methylation, phosphorylation, ubiquitylation and sumoylation.

Epigenetic and Gene Expression 

Transcription, translation and subsequent protein modification illustrate the transfer of genetic data from the archival copy of DNA to the short-lived messenger RNA, usually with subsequent generation of protein. Although all cells in an organism contain almost the same DNA, cell types and functions vary because of qualitative and quantitative distinctions in their gene expression. Therefore, control of gene expression is at the heart of differentiation and development. Epigenetic processes, containing DNA methylation, histone modification and various RNA-mediated processes, are thought to cause gene expression chiefly at the level of transcription; regardless, other steps in the procedure(for example, translation) may also be regulated epigenetically.

The three major mechanisms of epigenetics are DNA methylation, histone modification, and RNA regulation. 

1.DNA METHYLATION

DNA methylation is the greatly recognized mechanism of epigenetics. It generally involves a methyltransferase enzyme that helps with the addition of a methyl group on the fifth position of cytosine (C5). This addition arises primarily on cytosine-phosphate- guanine (CpG) dinucleotides. Nonetheless, non-CpG methylation arises as well. Analysis of DNA methylation is often conducted to enable an understanding of gene expression

2.HISTONE MODIFICATION

Histone modification is another traditional epigenetic mechanism. It includes several ways of altering histones by acetylation, methylation, phosphorylation, and other mechanisms that influence gene expression. 

3.RNA REGULATION

RNA signalling is believed to play a role in epigenetics by regulating chromatin structure. Researchers are exploring how mRNA and mainly non-coding RNA, such as long non-coding RNA, and micro RNA regulate gene expression.

Our Working Pathway

Features 

1.Accurate – All sequences are verified by sequencing and ensured error-free.

2.Flexible - Synthesize and clone any DNA sequences into our definitive vector or your vector of choice.

3.High purity - generate proteins to meet industry-leading purity specifications.

4.Long-term storage feasibility -Proteins are provided in a lyophilized form which ensures extra long terms of storage without significant protein mass or activity loss.

5.Talented Staff -Technical support is given by the very scientists that develop the assays.

Deliverables

√SDS-PAGE Data

√WB data

√The bioactivity Long-term data 

√The purified proteins are also shipped to our customers for them to test internally

References

1.Bird A (May 2007). "Perceptions of epigenetics". Nature. 447 (7143): 396–8.  

2.Hunter P (1 May 2008). "What genes remember". Prospect Magazine. Archived from the original on 1 May 2008. Retrieved 26 July 2012.

3.Reik W (May 2007). "Stability and flexibility of epigenetic gene regulation in mammalian development". Nature. 447 (7143): 425–32.