Bioactive™ Fc Receptor Protein Production Services

Our protein services are formulated to address the time-consuming and laborious generation of functional proteins and to simplify the process of generating recombinant proteins when seeking solutions for high throughput screening, functional validation, and structural biology research.

Fc receptor is a protein belonging to the immunoglobulin superfamily that provides for the protective functions of the immune system. Fc receptor is created on the surface of certain cells - including B lymphocytes, natural killer cells, macrophages, neutrophils and mast cells. Its name originated from its binding specificity for a part of an antibody recognized as the Fc portion. Fc receptors bind to antibodies that are connected to infected cells or invading pathogens. There are several kinds of Fc receptors based on the kind of antibody that they recognize. Their activity facilitates phagocytic or cytotoxic cells to eliminate microbes or infected cells by antibody-mediated phagocytosis or antibody-dependent cell-mediated cytotoxicity.

Fc-gamma receptors

All of the Fcγ receptors (FcγR) belong to the immunoglobulin superfamily and are the largely significant Fc receptors for inducing phagocytosis of opsonized (marked) microbes. This family comprises several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which alter in their antibody affinities due to their various molecular structure.

Fc-alpha receptors

Only one Fc receptor relates to the FcαR subgroup, which is named FcαRI (or CD89). FcαRI is established on the surface of neutrophils, eosinophils, monocytes, some macrophages (including Kupffer cells), and some dendritic cells. It is organized of two extracellular Ig-like domains and is a member of both the immunoglobulin superfamily and the multi-chain immune recognition receptor (MIRR) family. It signals by relating with two FcRγ signalling chains

Fc-epsilon receptors

Two categories of FcεR are known:

•the high-affinity receptor FcεRI is an element of the immunoglobulin superfamily (it has two Ig-like domains). FcεRI is established on epidermal Langerhans cells, eosinophils, mast cells and basophils. As a conclusion of its cellular distribution, this receptor plays an important role in controlling allergic responses. 

•the low-affinity receptor FcεRII (CD23) lives as a C-type lectin. FcεRII has multiple purposes as a membrane-bound or soluble receptor; it governs B cell growth and differentiation and blocks IgE-binding of eosinophils, monocytes, and basophils.

Protein Purification

Protein purification is important for the characterization of the function, structure, and interactions of proteins. Separation of the protein of interest from all impurities is generally the greatly challenging factor of protein purification. Through the variety of proteins and the customers with whom we have worked, our scientists have developed the agility to customize downstream processes to each particular case. Insoluble proteins can also be purified and refolded to their native state. We routinely cleanse proteins utilizing affinity chromatography, ion exchange, size-exclusion, hydrophobic interaction and other procedures.

Size-exclusion: separates proteins established on their differences in size.

Affinity chromatography: takes benefit of the reversible interactions between a protein and its particular ligand linked to the chromatography matrix.

Ion-exchange: manipulates the reversible interactions between a protein and a charged chromatography medium.

Hydrophobic interaction: influences the reversible interactions between a protein and a hydrophobic ligand bound to the chromatography matrix.

Protein or Antibody Labeling

The protein or antibody- method relates to the covalent attachment of molecules such as biotin, enzymes, and radioactive isotopes to specific areas of the target protein. BIC has formulated a procession of protein labelling techniques. The most prominent labels that our customers choose include FITC, enzyme conjugates (horseradish peroxidase and alkaline phosphatase), and biotin.

Endotoxin Removal

Endotoxins are major contaminants in commercially accessible proteins or biologically active substances. Endotoxin-contaminated protein specimens transfected into cells or injected into an animal host can initiate a strong immune response such as endotoxin shock, tissue injury, and even death.

Protein Interaction Analysis

Protein-protein interaction plays the main role in predicting the protein function of the target protein and the drug capacity of molecules. Although various proteins conduct their functions alone, the majority of proteins interact with others for a full range of biological actions.

In addition, BIC can provide tag-free protein purification, RNAase removal and large-scale protein output.

Advantages of Fc receptor protein products

Expressed by HEK293 Cells: post-translational change and proper protein folding

Multiple species: Human, Mouse, Cynomolgus/Rhesus macaque, Rat, Porcine, Rabbit, Feline, and Bovine, can be fully applied to different cross-species experiments

High purity: SDS-PAGE verification purity>95%, SEC-MALS verification purity>90%

Low endotoxin: ＜1.0 EU/µg

High stability: strict quality control to ensure high batch-to-batch consistency

Biotinylated Fc Receptor proteins labelled with AvitagTM offered: the labelling efficiency is high, and the labelling site is specific and clear, which is suitable for ELISA/SPR/BLI detection based on binding to streptavidin in the process of drug development and optimization procedure

Affinity verified by SPR & BLI: activity guaranteed, and strategies offered free of charge