Custom Protein Labeling Services
Custom Protein Labeling With Enzymes

Custom Protein Labeling With Enzymes

Seattle Genova offers enzyme-protein labeling conjugation offerings for signal producing molecules. Various reporter enzymes, which includes horseradish peroxidase (HRP), Alkaline phosphatase (AP) and plenty of others, may be connected to proteins via the use of various coupling chemistries, making sure the most retention of activity of each enzyme and protein. Seattle Genova provides services mentioned as follows:

1)Horseradish Peroxidase HRP-Protein Labeling 

2)Alkaline phosphatase AP- Protein labeling 

3)Beta-galactosidase Beta-gal- Protein labeling

These enzyme-antibody conjugates may be utilized in applications such as: Enzyme-related immunosorbent assays (ELISAs), Blotting techniques, In situ hybridization, Cytochemistry and Histochemistry detection systems.

Using various coupling chemistries, Seattle Genova will try and preserve the activity of the protein with the aid of using directing the placement of the label in order that it does not intrude with the protein binding site. Seattle Genova can assure the first-class of conjugates. For positive conjugations, it can be vital to modify/purify a protein and linkers to an enzyme.

Seattle Genova’s Modifications:

Aldehyde Introduction via Diol Oxidation

Aldehyde Introduction through Amine

Thiol Introduction through Traut's Reagent

Thiol Introduction through SATA

Disulfide Bond Reduction

Fab Preparation

F(ab')2 Preparation

Seattle Genova’s Cross-linkers:

Seattle Genova uses following cross-linkers to conjugate enzyme protein labeling.

NHS ester-Maleimide

Reductive Amination



Seattle Genova’s Service Highlights:


Seattle Genova’s Available Enzymes: 



Application (suitable for)

Alkaline Phosphatase (AP)

· Lower catalytic rate

· Greater stability

· Maintains linear reaction kinetics much longer


Horseradish Peroxidase (HRP) 

· Higher catalytic rate

· Good stability

· Maintains linear reaction kinetics

Antigen Detection Assays, ELISAs, Westerns Blots, and Immunohistochemistry

β-galactosidase (β-gal, beta-gal)

· Good catalytic activity

· Better stability

· Yield colored products on hydrolysis


Molecular biology research





Product is 1 mg up to several grams. 

A complete QC report.


Thank you for your interest in our Custom Protein Labeling With Enzymes services. Please complete the form below and we will contact you shortly.

Fields indicated by an * are required.

Please fill in the first name
Please fill in the last name
Please fill in the email
Please fill in the company
Please fill in the phone
Please fill in the postal code
Please fill in the city
Please agree to the agreement.