Custom Protein Labeling With Enzymes

Seattle Genova offers enzyme-protein labeling conjugation offerings for signal producing molecules. Various reporter enzymes, which includes horseradish peroxidase (HRP), Alkaline phosphatase (AP) and plenty of others, may be connected to proteins via the use of various coupling chemistries, making sure the most retention of activity of each enzyme and protein. Seattle Genova provides services mentioned as follows:

1)Horseradish Peroxidase HRP-Protein Labeling 

2)Alkaline phosphatase AP- Protein labeling 

3)Beta-galactosidase Beta-gal- Protein labeling

These enzyme-antibody conjugates may be utilized in applications such as: Enzyme-related immunosorbent assays (ELISAs), Blotting techniques, In situ hybridization, Cytochemistry and Histochemistry detection systems.

Using various coupling chemistries, Seattle Genova will try and preserve the activity of the protein with the aid of using directing the placement of the label in order that it does not intrude with the protein binding site. Seattle Genova can assure the first-class of conjugates. For positive conjugations, it can be vital to modify/purify a protein and linkers to an enzyme.

Seattle Genova’s Modifications:

●Aldehyde Introduction via Diol Oxidation

●Aldehyde Introduction through Amine

●Thiol Introduction through Traut's Reagent

●Thiol Introduction through SATA

●Disulfide Bond Reduction

●Fab Preparation

●F(ab')2 Preparation

Seattle Genova’s Cross-linkers:

Seattle Genova uses following cross-linkers to conjugate enzyme protein labeling.

➢NHS ester-Maleimide

➢Reductive Amination

➢Oxime

➢Dydrozone

Seattle Genova’s Service Highlights:

Seattle Genova’s Available Enzymes: 

Workflow:

Deliverables:

●Product is 1 mg up to several grams. 

●A complete QC report.