Seattle Genova offers enzyme-protein labeling conjugation offerings for signal producing molecules. Various reporter enzymes, which includes horseradish peroxidase (HRP), Alkaline phosphatase (AP) and plenty of others, may be connected to proteins via the use of various coupling chemistries, making sure the most retention of activity of each enzyme and protein. Seattle Genova provides services mentioned as follows:
1)Horseradish Peroxidase HRP-Protein Labeling
2)Alkaline phosphatase AP- Protein labeling
3)Beta-galactosidase Beta-gal- Protein labeling
These enzyme-antibody conjugates may be utilized in applications such as: Enzyme-related immunosorbent assays (ELISAs), Blotting techniques, In situ hybridization, Cytochemistry and Histochemistry detection systems.
Using various coupling chemistries, Seattle Genova will try and preserve the activity of the protein with the aid of using directing the placement of the label in order that it does not intrude with the protein binding site. Seattle Genova can assure the first-class of conjugates. For positive conjugations, it can be vital to modify/purify a protein and linkers to an enzyme.
Seattle Genova’s Modifications:
●Aldehyde Introduction via Diol Oxidation
●Aldehyde Introduction through Amine
●Thiol Introduction through Traut's Reagent
●Thiol Introduction through SATA
●Disulfide Bond Reduction
Seattle Genova’s Cross-linkers:
Seattle Genova uses following cross-linkers to conjugate enzyme protein labeling.
Seattle Genova’s Service Highlights:
Seattle Genova’s Available Enzymes:
Application (suitable for)
Alkaline Phosphatase (AP)
· Lower catalytic rate
· Greater stability
· Maintains linear reaction kinetics much longer
Horseradish Peroxidase (HRP)
· Higher catalytic rate
· Good stability
· Maintains linear reaction kinetics
Antigen Detection Assays, ELISAs, Westerns Blots, and Immunohistochemistry
β-galactosidase (β-gal, beta-gal)
· Good catalytic activity
· Better stability
· Yield colored products on hydrolysis
Molecular biology research
●Product is 1 mg up to several grams.
●A complete QC report.
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