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Protein kinase inhibitors are a well-established class of clinically useful drugs, particularly for the treatment of cancer. Measurement and prediction of kinase inhibitor selectivity will be important for the development of new multi-targeted kinase inhibitors.
SPR is label-free and capable of measuring real-time quantitative binding affinities and kinetics for kinases interacting with ligand molecules using relatively small quantities of materials and has potential to be medium-throughput.
It can be used with a wide range of kinase systems including GPCRs, which are the major molecular targets for current validated drugs and for foreseeable drug discovery.
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