Gastric Organoid Culture Service

Seattle Genova provides gastric organoids, derived cell aggregates cultured in three dimensions that grow similarly to their tissue of origin. The ability of our provided organoids to self-renewal and proliferative capacity could be maintained for the long term and expanded in many cases. Moreover, these organoids have opened new perspectives for multiple translation and clinical applications. Hence gastric organoids provide reliable markers for the early detection of gastric diseases.

Seattle Genova services

Fig: Gastric Organoid Provides In Vitro Model for The Study of Gastric Development (Seidlitz et al., 2021) 

Adult Stem Cells and Pluripotent Stem Cells Derived Gastric Organoids

Seattle Genova provides gastric organoids, which are derived from adult stem cell ADSCs as well as pluripotent stem cells PSCs. The main difference between both systems is the presence of mesenchymal cells within the PSC-derived organoid culture cultures. The AdCSs in our organoids generates specific cells from the tissue of origin, whereas PSCs can differentiate into any cell type. The PSCs and ADSCs mimic the functionality of in vitro gastric systems, enabling our organoid system to precisely represent the existing environment of gastric issues inside the human abdomen. 

Patient-Derived Gastric Cancer Organoids

Seattle Genova also offers gastric organoids as avatars to a cancer organoid. These gastric organoids can be utilized to predict the therapeutic responses to the drugs while establishing large patient-derived organoids in biobanks combined with drug screens which might help delineate novel therapeutic strategies. In addition, our services utilize four different groups of gastric PDO from which tissue samples were obtained for surgical resection and endoscopic, ultrasound- and computed tomography (CT)-guided biopsies or ascites punctures. The PDO biobank includes various cancers of different gastrointestinal origins and related tumors. 

Deliverables

Seattle Genova provides Gastric organoids with phenotypic and molecular characterizations. The individual PDOs show variant growth patterns characteristic to each line and document the organoid with compact cell clusters. Whereas access to the sequencing modalities allows molecular profiling by transcriptomic, genetic, and epigenetic analyses for the gastric organoids. 

References

Seidlitz, T., Koo, B. K., & Stange, D. E. (2021). Gastric organoids—an in vitro model system for the study of gastric development and road to personalized medicine. Cell Death & Differentiation, 28(1), 68-83.