Oesophageal Organoids Culture Service

Seattle Genova offers a wide range of esophageal 3D organoids, which are utilized to investigate the regulatory mechanisms of the esophagus in the context of diagnosis and analysis. Moreover, through our services, the genetic and pharmacologic manipulations establish proof of the utility of various esophageal organoids for future studies and personalized medicines in other esophageal diseases. Hence, we provide our organoids based on novelty and physiologically relevant to biological platforms.  

Seattle Genova Services

Seattle Genova services include esophageal organoids incorporating several distinct cell populations and rapidly dividing esophageal stem cells, facilitating the turnover cycle of oesophageal stem cells.

Fig: Schematic Concepts of Esophageal Organoids (Zheng et al., 2021) 

Organoids Derived from Epithelial Cell Lines

Seattle Genova generates various human and murine esophageal organoids derived explicitly from epithelial cell lines with genetic and pharmacologic modifications. The epithelial cell lines have intensive functional crosstalk between cytokines and notch signaling. Moreover, the squamous epithelial is also used for esophageal organoids. 

Organoids Derived from Pluripotent Stem Cells (PSCs)

Seattle Genova produces esophageal organoids derived from human and mouse pluripotent stem cells. Pluripotent cells can imitate and transform into any type of tissue in the body, and their ability to replicate the working of the esophagus. Hence, we utilize pluripotent stem cells to bioengineer the esophagus, stomach, colon, liver, and human intestine.

Patient Derived Esophageal Organoids  

Another esophageal organoid utilized by the Seattle Genova services is in the form of a patient-derived organoid. These PDOs serve as the treatment avatars, recapitulate the histopathological characteristics of the original tumors, and are validated by clinical pathologists.  

Deliverables

Seattle Genova provides esophagus organoidsi8 derived from a small number of cells isolated from tissues or cultures as starting materials. Our services of esophageal organoids give insight into the different roles of pathways and niche factors that play an essential role renewal, differentiation, and proliferation of esophageal cells. 

References

Zheng, B., Ko, K. P., Fang, X., Wang, X., Zhang, J., Jun, S., ... & Park, J. I. (2021). A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia. Science, 24(12), 103440.