Organoid Analysis With Flow Cytometry

Seattle Genova utilizes flow cytometry for the screening of organoids which investigates primarily changes in the surface marker expressions of organoids. The flow cytometry of our services demonstrated to be useful in detecting and analyzing the changes in the organoids composition, along with the influence of additional factors like surface markers expressions. Additional factors like growth conditions and identification of cell populations are detected through flow cytometry which requires appropriate controls.

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Work Flow of Flow Cytometry 

Seattle Genova utilizes large particle flow cytometers which provide automation for the deep analysis and assortment of organoids and tumor spheroids. The cell of organoids grows in clusters and communicates with each other and behaves differently than those cells which are grown as a monolayer or in suspensions. The iPSC-derived retinal epithelial organoids and T47D breast cancer tumor spheroids were able to discriminate at different stages of organoid formation as well as the expression level of fluorescent markers. The single cell of organoids is dispensed accurately and intact into the tubes or the multi-well plates. These multi-well plates are up to 38-well format and process not only quickly by quite efficiently as well.  The flow cytometry approach offers a high throughput manner that is used to assess the quality and sort the organoids which can be further used for transplantation, testing, and disease modeling. Utilizing the flow cytometry research approach, allowed for the cell-to-cell interactions to occur and provides the biological insights which otherwise missed while studying flat sheets of cells grown on a plastic surface or the cells grown in isolation.  

Example

Seattle Genova provides single-cell analysis of various matched organoid cultures and their native tissues through mass cytometry for 38 markers which were provided under a high resolution and was the representation of multiple mammary epithelial cell types inside the organoids. The flow cytometry was also able to demonstrate the expression of protein patterns of the tissue of origin which can be preserved in the culture.