Seattle Genova is pleased to offer an expanded and cost-effective molecular cloning services to boost your veterinary research. We have strong expertise for custom gene cloning and vector construction, shRNA, CRISPR/Cas9 synthesis, plasmid DNA and RNA purification, site-directed mutagenesis to introduce point mutations, small insertions, or deletions and other projects. We are capable to clone cDNA, ORFs from many species like dog, cat, sheep, cattle, horse, pig, chicken, duck and so on.
Custom Gene Cloning and Vector Construction
Cloning of cDNA, ORFs from any species in the lentiviral and AAV vector backbones
Protein-expression vectors for bacterial, and mammalian cell expression
We supply sequence-confirmed plasmid DNA and plasmid maps
Cloning using restriction enzyme digestion: sticky-end, blunt-end cloning
Cloning using PCR amplification: TA cloning or blunt-end cloning
Cloning using ligation free technology: use of the “in-fusion” method
Gene Synthesis & Subcloning
Synthesis of small fragments as well as long fragments, complimentary subcloning into pUC57, EcoRV site.
Cloning by RT-PCR
Isolated RNA/mRNA from any tissue is reverse transcribed and cloned into a plasmid-of-interest.
Viral Expression Vectors
Seattle Genova has an extensive collection of in-house AAV, Lentiviral and Retroviral vector backbones, with combination of heterologous envelopes, genes, promoters and tags.
Gene Editing Vectors
Vectors for CRISPR/cas9, homologous recombination, knock-in, knock-out, Cre, FLP, and Flox constructs for genome integration.
Inducible Expression Vectors
Conditional and inducible systems for a temporal and spatial controlled activation of genes including Tet-On, Tet-Off, and IPTG/tamoxifen-inducible.
Gene Knock-down Vectors
Cloning of miRNA, mi-shRNA and shRNA into a plasmid-of-interest- pol II or pol III-promoter systems are available for transient and stable expression.
Additions, deletions, substitutions, site-specific and random mutagenesis.
Promoter Change Service
Change the promoter in one of our vectors or a vector of your choice to any of the following: CMV, EF1a, PGK, UbC.
Tag Addition/Removal Service (≤30bp)
Addition/Removal of an N-terminal or C-terminal tag smaller than 30bp.
Reporter Addition/Removal Service
Addition, removal or switching of a GFP or RFP reporter in one of Lanhey Animal Sciences’ vectors or a vector of your choice.
Antibiotic Switching Service
Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of our vectors or a vector of your choice.
Amplification of customers’ plasmids from >10ug to 100mg.
The end-point plasmids released to investigator/used for vector production after we verify their sequence by digestion with restriction enzyme and Sanger sequencing.
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