Thyroid Organoid Culture Service

Seattle Genova introduces thyroid organoids to treat consequential hypothyroidism and thyroid disease. To maintain the proper level of thyroid hormones level in patients, regenerative medicines through organoid culture provide a potential therapy. The development of such treatment might replace the need for hormonal therapy. Our three-dimensional thyroid organoids offer a route for novel and improved treatments for various thyroid issues. Moreover, our services also capitalize on the capacity of stem cells to self-renewal and other primary thyroid cells. Hence our services play an essential role in using the thyroid organoid model for homeostasis, regeneration, and disease analysis.

Seattle Genova Services

Seattle Genova provides in vitro thyroid cultures primarily derived from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Fig: Generation and Differentiation of Adult Tissue-Derived Human Thyroid Organoids (Ogundipe et al.,2021)

Organoid Derived from Thyroid Follicles 

Seattle Genova utilizes the transplantation of organoids, which results in the generation and maturation of functional thyroid follicles. Moreover, the vascular formation after the transplantation might be beneficial for the pre-vascularization of the organoids. Our tissue-derived thyroid organoids combined with a vascular network by using biomaterials to generate 3D systems. The thyroid organoids should maintain the polarizing of thyrocytes, enable iodine transport, and facilitate hormone synthesis.     

Organoid Derived from Human Embryoid Stem Cells 

Seattle Genova utilizes thyroid derived from human ESC, which provides transcriptomics and histological analysis evidence. These, in return, generate a protocol that recapitulates the thyroid gland development, cell expansion, and follicular organization and results in T4 production. Moreover, we have utilized murine models for thyroid differentiation labeled with a fluorescent protein (GFP) in embryoid bodies (EBs).

Deliverables

Seattle Genova thyroids models open a new window for properly understating thyroid development processes and other variants causing congenital hypothyroidism. Moreover, our services could also consider as a potent screening tool to test the toxic effects of compounds, in particular endocrine disruptors. 

References

Ogundipe, V. M., Groen, A. H., Hosper, N., Nagle, P. W., Hess, J., Faber, H., ... & Coppes, R. P. (2021). Generation and differentiation of adult tissue-derived human thyroid organoids. Stem cell reports, 16(4), 913-925.