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Background
EBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 1998, derived from Aequorea victoria. EBFP is a blue fluorescent protein with an excitation peak of 380nm and an emission peak of 440nm in vitro. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Aequorea victoria GFP gene (UniProtKB:P42212), containing the mutations F64L, S65T, Y66H and Y145F (PMID:9525926). EBFP has low brightness and very poor photostability (PMID:20664080).
