Background
Renilla luciferase (RLuc) is commonly used as a reporter gene either on its own, or in conjunction with firefly and other beetle luciferases. Puromycin is an antibiotic derived from Streptomyces alboniger that induces chain termination during protein translation by releasing the ribosome. Puromycin is inactivated by puromycin N-Acetyltransferase encoded by the S. Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase.