Gene Accession

Q05127

Gene Alias

Polymerase cofactor VP35 (Ebola VP35) (eVP35)

Background

FUNCTION: Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling (PubMed:11027311). Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes (PubMed:9971816, PubMed:16495261, PubMed:24495995). Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes (By similarity). Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1 (PubMed:25741013). VP35-NP interaction controls the switch between RNA-bound NP and free NP and thus the switch between genome replication and genome packaging into the nucleocapsid (PubMed:25865894). Prevents establishment of cellular antiviral state, thereby suppressing host DC maturation (PubMed:26962215). Acts by inhibiting host RIGI activation both by shielding dsRNA from detection and by preventing PRKRA binding to RIGI (PubMed:23870315). Blocks virus-induced phosphorylation and activation of interferon regulatory factor 3/IRF3, a transcription factor critical for the induction of interferons alpha and beta (PubMed:12829834). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3 (PubMed:12829834). Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (PubMed:17065211). Increases PIAS1-mediated SUMOylation of IRF7, thereby repressing interferon transcription (PubMed:19557165). Also acts as a suppressor of RNA silencing by interacting with host DICER1, TARBP2/TRBP and PRKRA/PACT (By similarity). As a dimer, binds and sequesters dsRNA contributing to the inhibition of interferon production (By similarity). {ECO:0000250|UniProtKB:Q5XX07, ECO:0000250|UniProtKB:Q6V1Q9, ECO:0000250|UniProtKB:Q8JPY0, ECO:0000269|PubMed:11027311, ECO:0000269|PubMed:12829834, ECO:0000269|PubMed:16495261, ECO:0000269|PubMed:17065211, ECO:0000269|PubMed:19557165, ECO:0000269|PubMed:23870315, ECO:0000269|PubMed:24495995, ECO:0000269|PubMed:25741013, ECO:0000269|PubMed:25865894, ECO:0000269|PubMed:26962215, ECO:0000269|PubMed:9971816}.